Viral DNA/RNA Extraction Kit
This kit is suitable for the rapid extraction of high-purity viral DNA/RNA from samples such as nasopharyngeal swabs, environmental swabs, cell culture supernatants, and tissue homogenate supernatants. The kit is based on silica membrane purification technology that eliminates the need for using phenol/chloroform organic solvents or time-consuming alcohol precipitation to extract viral DNA/RNA of high quality. The nucleic acids obtained are free of impurities and ready for use in downstream experiments such as reverse transcription, PCR, RT-PCR, real-time PCR, next-generation sequencing (NGS), and Northern blot.
Storage conditions
Store at 15 ~ 25℃, and transport at room temperature
Components
|
Components |
100RXNS |
|
Buffer VL |
50 ml |
|
Buffer RW |
120 ml |
|
RNase-free ddH2 O |
6 ml |
|
FastPure RNA Columns |
100 |
|
Collection Tubes (2ml) |
100 |
|
RNase-free Collection Tubes(1 .5ml) |
100 |
Buffer VL: Provide an environment for lysis and binding.
Buffer RW: Remove residual proteins and other impurities.
RNase-free ddH2O: Elute DNA/RNA from the membrane in the spin column.
FastPure RNA Columns: Specifically adsorb DNA/RNA.
Collection Tubes 2 ml: Collect filtrate.
RNase-free Collection Tubes 1.5 ml: Collect DNA/RNA.
Applications
Nasopharyngeal swabs, environmental swabs, cell culture supernatants, and tissue homogenate supernatants.
Self-prepared Materials
RNase-free pipette tips, 1.5 ml RNase-free centrifuge tubes, centrifuge, vortex mixer, and pipettes.
Experiment Process
Perform all of the following steps in a biosafety cabinet.
1. Add 200 μl of the sample to an RNase-free centrifuge tube (make up with PBS or 0.9% NaCl in case of insufficient sample), add 500 μl of Buffer VL, mix well by vortexing for 15 – 30 sec, and centrifuge briefly to collect the mixture at the bottom of the tube.
2. Place FastPure RNA Columns in a Collection Tubes 2 ml. Transfer the mixture from Step 1 to FastPure RNA Columns, centrifuge at 12,000 rpm (13,400 × g) for 1 min, and discard the filtrate.
3. Add 600 μl of Buffer RW to FastPure RNA Columns, centrifuge at 12,000 rpm (13,400 × g) for 30 sec, and discard the filtrate.
4. Repeat Step 3.
5. Centrifuge the empty column at 12,000 rpm (13,400 × g) for 2 min.
6. Carefully transfer FastPure RNA Columns into a new RNase-free Collection Tubes 1.5 ml (provided in the kit),and add 30 – 50 μl of RNase-free ddH2O to the center of the membrane without touching the column. Allow to stand at room temperature for 1 min and centrifuge at 12,000 rpm (13,400 × g) for 1 min.
7. Discard FastPure RNA Columns. The DNA/RNA can be used directly for subsequent assays, or stored at -30~ -15°C for a short period or -85 ~-65°C for a longer period.
Notes
For research use only. Not for use in diagnostic procedures.
1. Equilibrate samples to room temperature in advance.
2. Viruses are highly infectious. Please ensure all necessary safety precautions are taken before the experiment.
3. Avoid repeated freezing and thawing of the sample, as this may lead to degradation or reduced yield of the extracted viral DNA/RNA.
4. Self-prepared equipment includes RNase-free pipette tips, 1.5 ml RNase-free centrifuge tubes, centrifuge, vortex mixer, and pipettes.
5. When using the kit, wear a lab coat, disposable latex gloves, and a disposable mask and use RNase-free consumables to minimize the risk of RNase contamination.
6. Perform all steps at room temperature unless otherwise specified.
Mechanism & Workflow
