Uracil DNA Glycoylase (Glycerol-free)
Description
Thermosensitive UDG (uracil-DNA glycosylase) can catalyze the hydrolysis of the uracil base of the DNA chain containing uracil and the N-glycosidic bond of the sugar-phosphate backbone to release free uracil. Compared with ordinary UDG enzymes, thermosensitive UDG enzymes avoid the possible residual activity of conventional UDG enzymes after inactivation, which may degrade dU-containing amplification products at room temperature. This product works at room temperature and is temperature sensitive and prone to inactivation.
Chemical Structure
Specification
Enzyme | Glycosylase |
Compatible Buffer | Storage Buffer |
Heat Inactivation | 50°C, 10 min |
Unit Definition | One unit (U) is defined as the amount of enzyme required to catalyze the hydrolysis of 1 μg dU-containing dsDNA in 30 minutes at 25°C. |
Applications
Remove dU-containing PCR product aerosol contamination.
Removal of uracil bases from single-stranded or double-stranded DNA
Shipping and Storage
Transportation: Ice packs
Storage Conditions: Store at -15℃ ~ -25℃
Shief life: 1years