RT-LAMP ColorImetric (Lyophilized Beads)
This product contains reaction buffer, RT-Enzymes Mix (Bst DNA polymerase and heat-resistant reverse transcriptase), lyophilized protectants and chromogenic dye components. The product is lyophilized ball type, using only with primers and templates. This kit provides a fast, clear visual detection of amplification, which negative reaction is indicated in red and positive reaction is indicated by a change to yellow.
Component
RT-LAMP Colormetric Master Mix (Lyophilized beads)
Applications
For DNA or RNA isothermal amplification.
Storage Conditions
Transported and stored at 2~ 8℃. The product is valid for 12 months.
Protocol
1.Take out the corresponding number Lyophilized beads powder according to the number of tests.
2.Prepare reaction mix
|
Component |
Volume |
|
RT-LAMP Colormetric Master Mix (Lyophilized beads) |
1 piece (2 beads) |
|
10 × Primer Mix a |
5 μL |
|
Templates DNA/ RNA b |
45 μL |
Notes:
1. 10×Primer Mix Concentration: 16 μM FIP/BIP, 2 μM F3/B3, 4 μM Loop F/B;
2. Nucleic acid templates are recommended to be dissolved using DEPC water.
3. Incubate at 65°C for 30-45mins, which can be extended appropriately according to color change Reaction time.
4. According to the naked eye, yellow was positive and red was negative.
Notes
1.The reaction temperature can be optimized between 62 ℃ and 68 ℃ according to the primer condition.
2.The packaged reagents should not be exposed to air for a long time.
3.The red and yellow discoloration reaction depend on the pH change of the reaction system, please do not use the containing Tris nucleic acid storage solution, recommended to use ddH2O stored nucleic acid.
4.The experiment shall be conducted in a standardized manner, including the preparation of reaction system, sample treatment and sample addition.
5.It is suggested to prepare reaction system in the ultra-clean table and add templates in the fume hood of other rooms to avoid false.
