RT-Lamp Colorimetric (In-Situ Lyophilization)

This product contains reaction buffer, RT- Enzymes Mix (Bst DNA polymerase  and heat- resistant reverse transcriptase), lyophilized protectants and chromogenic dye components.The product is in the tube bottom freeze-dried dosage form and only the primer and template can be added when used.

This kit provides a fast, clear visual detection of amplification, which negative reaction is indicated in red and positive reaction is indicated by a change to yellow.

Components

Applications

For DNA or RNA isothermal amplification.

Storage Conditions

Transported and stored at 2~ 8 ℃. The product is valid for 12 months.

Protocol

1. Take  out  the  corresponding  number  of  tube bottom  lyophilized  powder  according  to the number of tests.
2. Prepare reaction mix

Notes:

a. 10×Primer Mix Concentration: 16 μM FIP/BIP, 2 μM F3/B3, 4 μM Loop F/B;

b. Nucleic acid templates are recommended to be dissolved using DEPC water.

3. Incubate at 65°C for 30-45mins, which can be extended appropriately according to color change Reaction time.

4. Identify directly with eyes by colour, yellow was positive and red was negative.

Notes

1. The reaction temperature can be optimized between 62℃ and 68℃ according to the primer condition.  
2. The packaged reagents should not be exposed to air for a long time.
3. The red and yellow discoloration reaction depend on the pH change of the reaction system, please do not use the containing Tris nucleic acid storage solution, recommended to use ddH₂O stored nucleic acid.
4. The experiment shall be conducted in a standardized manner, including the preparation of reaction system, sample treatment and sample addition.
5. It is suggested to prepare reaction system in the ultra-clean table and add templates in the fume hood of other rooms to avoid false.