Ribonuclease III (RNase III)
Description
This product is ribonuclease III (RNase III) recombinantly expressed by E. coli. This specific exonuclease cleaves double-stranded RNA (dsRNA) and generates 12-35bp dsRNA fragments with 5'-PO4 and 3'-OH, 3' overhangs
Chemical Structure
Unit Definition
Activity unit definition: One unit refers to the amount of enzyme required to degrade 1 μg of
dsRNA to siRNA in a 50 μL reaction system at 37°C for 20 minutes.
Specification
| Test Items | Specifications |
| Exonuclease Activity | Releases＜ 0.1% of the total radioactivity |
| Non-Specific Dnase Activity | Not detectable |
| Protein Purity Assay(SDS-PAGE) | ≥ 95% |
| RNase Activity (Extended Digestion) | ＞90% of the substrate RNA remains intact |
Transportation and storage
Transportation: Dry ice
Storage: Store at -25~-15°C
Recommended re-test Life: 2 year
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