Reverse Transcriptase, Glycerol-free
Description
Reverse transcriptase 200U/μL,Glycerol-frees is a new reverse transcriptase obtained through genetic engineering technology. Compared with M-MLV () Reverse Transcriptase, its thermal stability It is greatly improved and can withstand reaction temperatures up to 65°C, suitable for reverse transcription of RNA templates with complex secondary structures. At the same time, the enzyme has enhanced affinity with the template, which is suitable for the reverse transcription of a small amount of template and low-copy genes. The ability of Reverse Transcriptase to synthesize full-length cDNA has also been improved, and cDNA up to 19.8 kb can be amplified. Reverse Transcriptase, 200 U/μL, Glycerol-free third-generation thermostable reverse transcriptase (glycerol-free version) can be used to prepare lyophilized preparations, lyophilized RT-LAMP reagents, etc.
Chemical structure
Specification
Test Items |
Specification |
Result |
(SDS PAGE) Purity of Enzyme Stock(SDS PAGE) |
≥95% |
Pass |
Endonuclease Activity |
Not detected |
Pass |
Exodulease Activity |
Not detected |
Pass |
Rnase Activity |
Not detected |
Pass |
Residual E.coli DNA |
<1 copy/60U |
Pass |
Functional Assay-System |
90%≤110% |
Pass |
Applications
Freeze-dried Kit
Lyophilizable RT-LAMP kit.
Shipping and Storage
Transportation: Ice packs
Storage Conditions: Store at -30~-15℃.
Shief life: 18Months