One Step RT-qPCR probe kit
Description
U+ One Step RT-qPCR Probe Kit (Glycerol-free) is a glycerol-free one-step RT-qPCR reagent using RNA as a template (such as RNA virus), which is suitable for the development and design of lyophilized products. This product integrates the superior performance of one-step dedicated Reverse Transcriptase and hot-start Champagne Taq DNA Polymerase, with well-optimized freeze-drying chemical buffer, which has better amplification efficiency, balance and specificity, and is compatible with different freeze-drying processes.In addition, the dUTP/UDG anti-pollution system is introduced into the reagent, which can work at room temperature, eliminate the impact of amplification product contamination on qPCR, and ensure the accuracy of the results.
Basic Principles of RT-qPCR
Specification
|
Test Items |
Specification |
Result |
|
(SDS PAGE) Purity of Enzyme Stock(SDS PAGE) |
≥95% |
Pass |
|
Endonuclease Activity |
Not detected |
Pass |
|
Exodulease Activity |
Not detected |
Pass |
|
Rnase Activity |
Not detected |
Pass |
|
Residual E.coli DNA |
＜1copies/60 |
Pass |
|
Functional Assay-System |
90%≤110% |
Pass |
Components
|
Components |
100rxns |
1,000rns |
5,000 rxns |
|
RNase-free ddH2O |
2*1ml |
20ml |
100ml |
|
5*one step mix |
600μl |
6*1ml |
30ml |
|
One step enzyme mix |
150μl |
2*750μl |
7.5ml |
|
50* ROX reference Dye 1 |
60μl |
600μl |
3*1ml |
|
50* ROX reference Dye 2 |
60μl |
600μl |
3*1ml |
a. One-Step Buffer includes dNTP Mix and Mg2+.
b. Enzyme Mix mainly contains reverse
transcriptase, Hot Start Taq DNA polymerase (antibody modification) and RNase inhibitor.
c. Used to correct the error of fluorescene sinals between diferent wells.
c. ROX: You need to choose the calibration according to the model of the testing instrument.
Applications
QPCR detection
Shipping and Storage
Transportation: Ice packs
Storage Conditions: Store at -20℃.
Shief Life: 18months
