Hotstart Taq DNA Polymerase (5u/ul)
Taq DNA Polymerase is a hot start DNA polymerase with double blocking by double antibodies.This product not only blocks the 5′→3′ polymerase activity of Taq DNA polymerase,but also blocks the 5′→3′exonuclease activity. Heating for 30 seconds at the pre-denaturation temperature can completely inactivate the antibody and release DNA polymerase activity and exonuclease activity. The double blocking characteristic can not only effectively prevent the nonspecific amplification caused by mismatch or primer dimer, but also effectively inhibit the decline of fluorescence signal caused by probe degradation, so as to make the in vitro detection reagent more stable during transportation or use at room temperature.
Components
|
Component |
HC1012B (250U) |
HC1012B (1000U) |
HC1012B (10000U) |
HC1012B (25000U) |
|
Taq DNA Polymerase(5 U/μL) |
50 μL |
200 μL |
2 mL |
5 mL |
Storage Condition
The product is shipped with dry ice and can be stored at -25°C~-15°C for 2 years.
Specifications
|
Polymerase |
Taq DNA Polymerase |
|
Purity |
≥ 95% (SDS-PAGE) |
|
Hot Start |
Built-in Hot Start |
|
Reaction Speed |
Standard |
|
Exonuclease Activity |
5′→3′ |
Instructions
Reaction Setup
|
Components |
Volume (μL) |
Final Concentration |
|
2× Buffer a |
25 |
1× |
|
Primer/Probe mix b |
× |
0.1 μmol/L-0.5 μmol/L |
|
Hotstart Taq Polymerase (5U/μL) |
1.2 |
0.12 U/μL |
|
DNA Template c |
× |
0.1-100 ng |
|
ddH2O |
Up to 50 |
- |
Notes:
1) According to the specific experimental application, it is needed to prepare the corresponding reaction buffer.
2) The amount of DNA and the concentration of probes or primers are recommended concentrations. The optimal concentration can be adjusted according to the specific experimental conditions.
Thermal cycling protocol
|
Step |
Temperature (°C) |
Time |
Cycles |
|
Pre-denaturation |
95 ℃ |
5mins |
1 |
|
Denaturation |
95 ℃ |
15 sec |
45 |
|
Annealing / Extension |
60 ℃ a |
30 sec b |
Notes:
1) The reaction temperature is adjusted according to the Tm value of the designed primers.
2) Different qPCR instruments need different fluorescence signal acquisition time, please set according to the shortest time limit.
Notes
Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety!
