M-MLV Reverse Transcriptase (Glycerol free)
A lyophilizable Reverse Transcriptase. It can be applied to downstream lyophilization technology while maintaining the great reverse transcription performance and stability. This product does not contain excipients, please add your own as needed.
Components
|
Component |
HC2005A-01 (10,000U) |
HC2005A-02 (40,000U) |
|
Reverse Transcriptase (Glycerol Free) (200U/μL) |
50 μL |
200 μL |
|
5 × Buffer |
200 μL |
800 μL |
Application:
It is applicable for one-step RT-qPCR reactions.
Storage Condition
Store at -30 ~ -15°C and transport at ≤0°C.
Unit Definition
One unit (U) is defined as the amount of enzyme that incorporates 1 nmol of dTTP into acid-insoluble material in 10mins at 37°C, with Poly(rA)·Oligo (dT) as the template/primer.
Notes
For research use only. Not for use in diagnostic procedures.
1.Please keep the experiment area clean; Wear disposable gloves and masks; Use RNase-free consumables such as centrifuge tubes and pipette tips.
2.Keep RNA on ice to avoid degradation.
3.High quality RNA templates are recommended to achieve high efficiency reverse transcription.
