LAMP Colorimetric Master Mix (Lyophilized Beads)

LAMP Colorimetric Master Mix (Lyophilized Beads) is all-in-one LAMP kit,Mix, add template, and detect with color change. High efficiency, sensitivity, and easy to use in isothermal DNA/RNA amplification.

Cat No: HCB5209A

Package: 96RXN/960RXN/9600RXN

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Product Description

Product details

Product Tags

This product contains reaction buffer, Enzymes Mix of Bst DNA polymerase, lyophilized protectants and chromogenic dye components. The reaction buffer contains Mg²⁺, dNTP and other components necessary for amplification components. The product is lyophilized in pellet form, and only primers and templates need to be added for use.

This kit provides a fast, clear visual detection of amplification, which negative reaction is indicated in red and positive reaction is indicated by a change to yellow.

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Components

LAMP Colorimetric Master Mix (Lyophilized beads) : 96T/960T/9600T

Applications

For DNA isothermal amplification.

Storage Conditions

Transported and stored at 2~ 8 ℃. The product is valid for 12 months.

Protocol

1. Take out the corresponding number Lyophilized beads according to the number of tests.

2. Prepare reaction mix

Component	Volume
LAMP Colorimetric Master Mix (Lyophilized beads)	1 piece (2 beads)
10 × Primer Mix ^a	5 μL
Templates DNA/ RNA^b	Up to 50 μL
Total	50 μL

Notes:

a. 10×Primer Mix Concentration: 16 μM FIP/BIP, 2 μM F3/B3, 4 μM Loop F/B;

b. Nucleic acid templates are recommended to be dissolved using DEPC water.

3. Incubate at 65°C for 30-45mins, which can be extended appropriately according to color change Reaction time.

4. According to the naked eye, yellow was positive and red was negative.

Notes

1. The reaction temperature can be optimized between 62 ℃ and 68 ℃ according to the primer condition.

2. The packaged reagents should not be exposed to air for a long time.

3. The red and yellow discoloration reaction depend on the pH change of the reaction system, please do not use the containing Tris nucleic acid storage solution, recommended to use ddH₂O stored nucleic acid.

4. The experiment shall be conducted in a standardized manner, including the preparation of reaction system, sample treatment and sample addition.

5. It is suggested to prepare reaction system in the ultra-clean table and add templates in the fume hood of other rooms to avoid false.