Hexokinase (HK)
Description
Use Hexokinase for the determination of D-glucose, D-fructose, and D-sorbitol in food or biological research samples. The enzyme is also used for the assay of other saccharides which are convertible to glucose or fructose, and is therefore useful in the assay of many glycosides.
If Hexokinase is used in combination with glucose-6-phosphate dehydrogenase (G6P-DH)* (assays glucose6-phosphate formed by Hexokinase), samples should not be of high phosphate concentrations as G6P-DH is competitively inhibited by phosphate.
Chemical Structure
Reaction Principle
D-Hexose + ATP --Mg2+→ D-Hexose-6-phosphate + ADP
Specification
| Test Items | Specifications |
| Description | White to little yellow
amorphous powder, lyophilized |
| Activity | ≥30U/mg |
| Purity(SDS-PAGE) | ≥90% |
| Solubility(10mg powder/ml) | Clear |
| Proteases | ≤0.01% |
| ATPase | ≤0.03% |
| Phosphoglucose isomerase | ≤0.001% |
| Creatine phosphokinase | ≤0.001% |
| Glucose-6-Phosphate dehydrogenase | ≤0.01% |
| NADH/NADPH oxidase | ≤0.01% |
Transportation and storage
Transportation: Ambient
Storage : Store at -20°C(Long term), 2-8°C (short term)
Recommended re-test Life: 1 year
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