DNA Extraction kit(Automated purification with column method)
The kit is designed for replace QIAamp 96 DNA QIAcube HT Kit, Kit facilitates streamlined, automated purification of total DNA encompassing genomic, mitochondrial, and pathogenic strands from diverse sources such as blood, cells, and tissue specimens. This kit is adept at concurrently processing 24–96 samples of 200 μl in volume to be used on QIAcube HT System. This DNA extraction kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the 96 Well Binding Plate. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, and is free of proteins, nucleases, and other contaminants or
inhibitors. The entire procedure requires no organic extraction or alcohol precipitation. The purified DNA (approximately 20-30 kb) is ready for use in enzymatic reactions, such as PCR, or storage at – 15°C to –30°C.
Components
|
GST Buffer |
50 ml |
Alternative to Buffer ATL |
|
GSB Buffer |
30 ml |
Alternative to Buffer VXL |
|
CFL Buffer1(Add isopropanol) |
60 ml (40 ml) |
Alternative to Buffer ACB |
|
Proteinase K2 powder (Add ddH2O) |
65 mg x2 (3 ml) |
Shipping at room temperature Long-term storage at 4ºC |
|
W1 Buffer |
220 ml |
Alternative to Buffer AW1 |
|
Wash Buffer3(Add ethanol) |
50 ml (200 ml) |
Alternative to Buffer AW2 |
|
Elution Buffer |
120 ml |
Alternative to Buffer AE |
|
gDNA 96 Well Binding Plates |
2 pcs |
Alternative to QIAamp 96 plates |
1. Before using for the first time, add isopropanol as indicated on the bottle to obtain a working solution.
2. Add ddH2O to Proteinase K (see the bottle label for volume) then vortex to ensure it is completely dissolved. Check the box on the bottle. For extended periods, the ddH2O and Proteinase K mixture should be stored at 4ºC.
3. Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.
Note
GSB Buffer, CFL Buffer and W1 Buffer contains chaotropic salt. During operation, always wear a lab coat, disposable gloves, protective goggles and (anti-fog) procedure mask.
Storage conditions
Are stable for 1 year at room temperature (15–25°C). After adding ddH2O to the Proteinase K powder, the Proteinase K solution should be stored at 4ºC.
Additional Requirements
Isopropanol, absolute ethanol, phosphatebuffered saline (PBS), ddH2O, microcentrifuge tubes, pipette tips , QIAcube HT Instrument, QIAcube HT Prep Manager Software, QIAcube HT Reagent Troughs, vortexer.
For tissue samples: Tissue disruption system such as the TissueLyser II , 4 mm stainless steel beads, centrifuge.
Notes before use
Components
|
GST Buffer |
50 ml |
Alternative to Buffer ATL |
|
GSB Buffer |
30 ml |
Alternative to Buffer VXL |
|
CFL Buffer1(Add isopropanol) |
60 ml (40 ml) |
Alternative to Buffer ACB |
|
Proteinase K2 powder (Add ddH2O) |
65 mg x2 (3 ml) |
Shipping at room temperature Long-term storage at 4ºC |
|
W1 Buffer |
220 ml |
Alternative to Buffer AW1 |
|
Wash Buffer3(Add ethanol) |
50 ml (200 ml) |
Alternative to Buffer AW2 |
|
Elution Buffer |
120 ml |
Alternative to Buffer AE |
|
gDNA 96 Well Binding Plates |
2 pcs |
Alternative to QIAamp 96 plates |
1. Before using for the first time, add isopropanol as indicated on the bottle to obtain a working solution.
2. Add ddH2O to Proteinase K (see the bottle label for volume) then vortex to ensure it is completely dissolved. Check the box on the bottle. For extended periods, the ddH2O and Proteinase K mixture should be stored at 4ºC.
3. Add absolute ethanol (see the bottle label for volume) to Wash Buffer then mix by shaking for a few seconds. Check the box on the bottle. Be sure and close the bottle tightly after each use to avoid ethanol evaporation.
Note
GSB Buffer, CFL Buffer and W1 Buffer contains chaotropic salt. During operation, always wear a lab coat, disposable gloves, protective goggles and (anti-fog) procedure mask.
Storage conditions
Presto™ gDNA 96 Well Binding Plates and all buffers are stable for 1 year at room
temperature (15–25°C).
After adding ddH2O to the Proteinase K powder, the Proteinase K solution should be stored at 4ºC.
Additional Requirements
Isopropanol, absolute ethanol, phosphate-buffered saline (PBS), ddH2O, microcentrifuge tubes, pipette tips , QIAcube HT Instrument, QIAcube HT Prep Manager Software, QIAcube HT Reagent Troughs, vortexer.
For tissue samples: Tissue disruption system such as the TissueLyser II , 4 mm stainless steel beads, centrifuge.
Notes before use
If handling fewer than 96 samples, you can reuse unused sections of Presto™ gDNA 96 Well Binding Plates, 96 deep well plates and Elution microtubes up to two times. Always ensure to cover the unused wells of the gDNA 96 Well Binding Plate and 96 deep well plate with a tape sheet.
Prepare buffers appropriately at the first use. Add ddH2O to Proteinase K powder and store the Proteinase K solution at 4ºC. Add isopropanol to the CFL Buffer as indicated on the bottle to obtain a working solution. Add absolute ethanol to the Wash Buffer as indicated on the bottle to obtain a working solution.
