ALP（Alkaline Phosphatase）
Alkaline Phosphatase is derived from a recombinant E.coli strain that carries the TAB5 gene. The enzyme catalyzes the dephosphorylation of 5´ and 3´ ends of DNA and RNA phosphomonoesters. Also, it hydrolyses ribose, as well as deoxyribonucleoside triphosphates (NTPs and dNTPs). TAB5 Alkaline Phosphatase acts on 5´ protruding, 5´ recessed and blunt ends. The Phosphatase can be used in many molecular biology applications, such as cloning or probe end labeling to remove the phosphorylated ends of DNA or RNA.
Preparation and specification
|
Appearance |
Transparent liquid |
|
Specific activity |
≥5000 U/mg |
|
Concentration |
≥10 mg/mL |
Properties
|
Source |
Recombinant fungi |
|
EC number |
3.1.3.1 |
|
Molecular weight |
140 kDa (SDS-PAGE) |
|
Buffer system |
30 mM TEA pH7.6, 5 mM MgCl2， 0. 1 mM ZnCl2, 3 M NaCl |
|
Activators |
Mg2+, Zn2+ |
|
Optimum pH |
9.8 |
Storage conditions
Store at 2-8 ℃, valid for 2 years.
Storage Buffer
10 mM Tris-HCl(pH 7.4，25℃), 1 mM MgCl2 , 0.01 mM ZnCl2, 50% glycerol.
Application
1. Alkaline phosphatase coupled to a protein (antibody, streptavidin, etc.) specifically recognizes the target molecule and can be used in ELISA, WB and histochemical assays;
2. Alkaline phosphatase can be used to dephosphorylate the 5′-end of DNA or RNA to prevent self-association;
3. The above dephosphorylated DNA or RNA can be labeled with radiolabeled phosphate (via T4 polynucleotide kinase).
Notes
The material is only used in in vitro diagnostic products, not in human or in vivo experiments.
