2×Rapid Taq Super Mix
Cat No: HCR2016A
2×Rapid Taq Super Mix is based on the modified Taq DNA Polymerase, adding strong extension factor, amplification enhancement factor and optimized buffer system, with super high amplification efficiency. The amplification speed of complex templates such as genome within 3 kb reaches 1-3 sec/kb, and that of simple templates like plasmids within 5 kb reaches 1 sec/kb. This product can greatly save PCR reaction time. At the same time, mix contains dNTP and Mg2+, which can be amplified only by adding primers and templates, which also greatly simplifies the operation steps of the experiment. Furthermore, mix contains electrophoretic indicator dye, which can be directly electrophoresis after the reaction. The protective agent in this product makes the mix maintain stable activity after repeated freeze and thawing. The 3’-end band A of the PCR product can be easily cloned into the T vector.
Components
2×Rapid Taq Super Mix
Storage Conditions
PCR Master Mix products should be stored at -25~-15℃ for 2 years.
Specifications
|
Product specification |
Rapid Taq Super Mix |
|
Concentration |
2× |
|
Hot Start |
Built-in Hot Start |
|
Overhang |
3′-A |
|
Reaction speed |
Rapid |
|
Size (Final Product) |
Up to 15 kb |
|
Conditions for transportation |
Dry ice |
Instructions
1. Reaction System (50 μL)
|
Components |
Size (μL) |
|
Template DNA* |
suitable |
|
Forward primer (10 μmol/L) |
2.5 |
|
Reverse primer (10 μmol/L） |
2.5 |
|
2×Rapid Taq Super Mix |
25 |
|
ddH2O |
to 50 |
2. Amplification Protocol
|
Cycle steps |
Temperature (°C) |
Time |
Cycles |
|
Predenaturation |
94 |
3 min |
1 |
|
Denaturation |
94 |
10 sec |
28-35 |
|
Annealing |
60 |
20 sec |
|
|
Extension |
72 |
1-10 sec/kb |
Recommended usage of different templates:
|
Type of template |
Segment usage range (50 μL reaction system) |
|
Genomic DNA or E. coli liquid |
10–1,000 ng |
|
Plasmid or viral DNA |
0.5-50 ng |
|
cDNA |
1-5 µL (no more than 1/10 of the total volume of the PCR reaction) |
|
Recommended usage of different templates |
|
Notes:
1. Reagent use: fully thaw and mix before use.
2. Annealing temperature: The annealing temperature is the universal Tm value, and can also be set 1-2℃ lower than the primer Tm value.
3. Extension speed: Set 1 sec/kb for complex templates such as genome and E. coli within 1 kb; set 3 sec/kb for complex templates such as 1-3 kb genome and E. coli; set 10 sec/kb for complex templates over 3 kb genome and E. coli. You can set the value to 1 sec/kb for a simple template such as a plasmid less than 5 kb, 5 sec/kb for a simple template such as a plasmid between 5 and 10 kb, and 10 sec/kb for a simple template such as a plasmid larger than 10 kb.
Notes
1. For your safety and health, please wear lab coats and disposable gloves for operation.
2. This product is for research use ONLY!
